Proteomic Characterization of Spontaneous Stress-Induced In Vitro Apoptosis of Human Acute Myeloid Leukemia Cells; Focus on Patient Heterogeneity and Endoplasmic Reticulum Stress

In vitro culture is widely used for characterization of primary human acute myeloid leukemia (AML) cells, but even when using optimized handling and conditions the AML cells show spontaneous in apoptosis with a gradual decrease cell viability during culture. The extent this stress-induced varies between patients, high degree associated pre-culture BCL2 levels together low BAX Heat Shock Proteins 30 90. We compared global proteomic profiles ongoing patients (i.e., less extensive versus apoptosis) after 48 h identified 7902 proteins, only 276 proteins differed significantly >25% viable cells; 192 upregulated 84 downregulated peptides) Protein interaction network analysis based on these protein three networks that included 18 proteins; most were localized to endoplasmic reticulum several them are involved or altered process stress/unfolded stress response. To conclude, heterogeneous regard response cellular stress, difference regulation differences induction and/or unfolded